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KMID : 0613820180280101147
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Journal of Life Science
2018 Volume.28 No. 10 p.1147 ~ p.1155
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Agaricus blazei Mycelial Liquid Culture Extract Containing Eritadenine Improves Benign Prostatic Hyperplasia-related Biochemical Markers in RWPE-1 Cells through Anti-inflammatory and Anti-oxidative Actions
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Ha Yeong-Lae
Moon Yeon-Kyu
Kim Na-Hyun
Heo Jeong-Doo
Cho Min-Jung
Kim Ye-Ra
Kim Young-Suk
Kim Jeong-Ok
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Abstract
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Agaricus blazei mycelial liquid culture extract (ABMLCE) promoted the production of testosterone (TS) in TM-3 mouse Leydig testis cells. Now, we report that ABMLCE containing eritadenine (EA) as a minor constituent (15.3 mg/100 g) reduced 5α-reductase 2 (5α-R2) enzyme activity and dihydrotestosterone (DHT) content which are key constituents for the benign prostatic hyperplasia (BPH) inductions. RWPE-1 prostate cells were grown in a Keratinocyte serum-free medium (K-SFM) containing ABMLCE (0~50 ppm), EA (0~10 ppm,), and finasteride (FS 10 μM: a positive control) in a 24-well plate for 24 hr. Supernatants collected from cell-cultured media were used for the assay of 5α-R2, superoxide dismutase (SOD), catalase (CAT) and cyclooxygenase-2 (COX-2) enzyme activities, and for TS, DHT, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) contents by their assay kits. The 5α-R2 activity and DHT content were proportionally reduced (p<0.05) to concentrations of ABMLCE. The SOD and CAT enzyme activities were significantly (p<0.05) elevated concomitant with ABMLCE concentrations, while COX-2, TNF-α and IL-1β showed reverse results (p<0.05). Similarly, the effects of EA were similar to those of ABMLCE. Efficacies of ABMLCE 50 ppm and EA 10 ppm in 5α-R2 and DHT reduction were similar to those of 10 μM FS. These results suggest that ABMLCE and EA reduced 5α-R2 and DHT through their anti-inflammatory and anti-oxidative actions. This implies that ABMLCE containing EA could be a beneficial material in the cure of BPH in humans.
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KEYWORD
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Agaricus blazei mycelial liquid culture extract, benign prostatic hyperplasia, eritadenine, 5α-reductase 2, RWPE-1 cell
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